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Western Blot analysis of A549 cells using LAT Polyclonal Antibody
Western Blot analysis of A549 cells using LAT Polyclonal Antibody
Western Blot analysis of A549 cells using LAT Polyclonal Antibody

LAT Antibody

CSB-PA003139
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLAT
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Overview

  • Supplier
    Cusabio
  • Product Name
    LAT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27040
  • Target name
    LAT
  • Target description
    linker for activation of T cells
  • Target synonyms
    36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; IMD52; LAT1; linker for activation of T cells, transmembrane adaptor; linker for activation of T-cells family member 1; p36-38; pp36
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43561
  • Protein Name
    Linker for activation of T-cells family member 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LAT using anti-LAT antibody (A01654-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAT antigen affinity purified polyclonal antibody (Catalog # A01654-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAT at approximately 37 kDa. The expected band size for LAT is at 28 kDa.
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