Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_T-45026_20230505_WB_M_R_23050918_780.webp "Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_T-45026_20230512_IHC-P_23060622_227.webp "LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_T-45026_20230804_ICC_IF_23080901_505.webp "LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_45089_20231006_ICC_IF_23102401_700.webp "LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min. Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_45481_20240816_WB_TPM_watermark_24122400_296.webp "Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_45481_20240802_WB_shRNA_watermark_24122400_741.webp "Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638556/GTX638556_45635_20250103_WB_TPM_watermark_25102220_791.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
LAT1 / SLC7A5 antibody [HL2353]
GTX638556
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC7A5
Overview
- SupplierGeneTex
- Product NameLAT1 / SLC7A5 antibody [HL2353]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2353
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8140
- Target nameSLC7A5
- Target descriptionsolute carrier family 7 member 5
- Target synonyms4F2LC, CD98, D16S469E, E16, LAT1, MPE16, large neutral amino acids transporter small subunit 1, 4F2 light chain, CD98 light chain, L-type amino acid transporter 1, integral membrane protein E16, sodium-independent neutral amino acid transporter LAT1, solute carrier family 7 (amino acid transporter light chain, L system), member 5, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
- HostRabbit
- IsotypeIgG
- Protein IDQ01650
- Protein NameLarge neutral amino acids transporter small subunit 1
- Scientific DescriptionEnables L-leucine transmembrane transporter activity; L-tryptophan transmembrane transporter activity; and thyroid hormone transmembrane transporter activity. Involved in carboxylic acid transport; thyroid hormone transport; and xenobiotic transport. Located in cytosol; intracellular membrane-bounded organelle; and plasma membrane. Is integral component of membrane. Part of amino acid transport complex; apical plasma membrane; and microvillus membrane. [provided by Alliance of Genome Resources, Apr 2022]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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