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Western Blot analysis of RAT-MUSCLE cells using LATS1/2 Polyclonal Antibody
Western Blot analysis of RAT-MUSCLE cells using LATS1/2 Polyclonal Antibody
Western Blot analysis of RAT-MUSCLE cells using LATS1/2 Polyclonal Antibody

LATS1/LATS2 Antibody

CSB-PA003141
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetLATS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LATS1/LATS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9113
  • Target name
    LATS1
  • Target description
    large tumor suppressor kinase 1
  • Target synonyms
    WARTS, wts, serine/threonine-protein kinase LATS1, LATS (large tumor suppressor, Drosophila) homolog 1, LATS, large tumor suppressor, homolog 1, WARTS protein kinase, h-warts, large tumor suppressor homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95835
  • Protein Name
    Serine/threonine-protein kinase LATS1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LATS1 using anti-LATS1 antibody (A01051-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LATS1 antigen affinity purified polyclonal antibody (Catalog # A01051-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LATS1 at approximately 150KD. The expected band size for LATS1 is at 127KD.
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