Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE GTX116080 diluted at 1:1000
![LC3B antibody [N1C3] (GTX116080) detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody [N1C3] (GTX116080) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116080/GTX116080_40212_20150212_FACS_w_23060519_656.webp "LC3B antibody [N1C3] (GTX116080) detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody [N1C3] (GTX116080) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.")
![LC3B antibody [N1C3] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody [N1C3] (GTX116080) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116080/GTX116080_40212_IFA_w_23060519_928.webp "LC3B antibody [N1C3] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody [N1C3] (GTX116080) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE GTX116080 diluted at 1:1000
LC3B antibody [N1C3]
GTX116080
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP1LC3B
Overview
- SupplierGeneTex
- Product NameLC3B antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.37 mg/ml
- ConjugateUnconjugated
- Gene ID81631
- Target nameMAP1LC3B
- Target descriptionmicrotubule associated protein 1 light chain 3 beta
- Target synonymsATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule-associated protein 1 light chain 3 beta, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, autophagy-related ubiquitin-like modifier LC3 B, microtubule-associated proteins 1A/1B light chain 3B
- HostRabbit
- IsotypeIgG
- Protein IDQ9GZQ8
- Protein NameMicrotubule-associated protein 1 light chain 3 beta
- Scientific DescriptionThe product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Marino N, German R, Rao X, et al. Upregulation of lipid metabolism genes in the breast prior to cancer diagnosis. NPJ Breast Cancer. 2020,6:50. doi: 10.1038/s41523-020-00191-8Read this paper
- Li Y, Chen C, Xu D, et al. Effective Theranostic Cyanine for Imaging of Amyloid Species in Vivo and Cognitive Improvements in Mouse Model. ACS Omega. 2018,3(6):6812-6819. doi: 10.1021/acsomega.8b00475Read this paper
Datasheet
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