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LCA3 / SPATA7 Antibody

LS-C401061
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSPATA7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LCA3 / SPATA7 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55812
  • Target name
    SPATA7
  • Target description
    spermatogenesis associated 7
  • Target synonyms
    HEL-S-296, HSD-3.1, HSD3, LCA3, RP94, spermatogenesis-associated protein 7, epididymis secretory protein Li 296, epididymis secretory sperm binding protein, spermatogenesis-associated protein HSD3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPATA7 using anti-SPATA7 antibody (A08310-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA7 antigen affinity purified polyclonal antibody (Catalog # A08310-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA7 at approximately 68 kDa. The expected band size for SPATA7 is at 68 kDa.
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