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ProductsBack/LCP1 / L-Plastin Antibody (aa277-291)

LCP1 / L-Plastin Antibody (aa277-291)

LS-C742525
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLCP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LCP1 / L-Plastin Antibody (aa277-291)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 70kDa band observed in lysates of cell lines Daudi and U937 (calculated MW of 70.3kDa according to NP_002289.2). Recommended concentration: 0.5-3 ug/ml.. Peptide-ELISA (1:16000), WB (0.5 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 70kDa band observed in lysates of cell lines Daudi and U937 (calculated MW of 70.3kDa according to NP_002289.2). Recommended concentration: 0.5-3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3936
  • Target name
    LCP1
  • Target description
    lymphocyte cytosolic protein 1
  • Target synonyms
    CP64, HEL-S-37, L-PLASTIN, LC64P, LPL, PLS2, plastin-2, L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P), LCP-1, Lymphocyte cytosolic protein-1 (plasmin), bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)), epididymis secretory protein Li 37
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LCP1 using anti-LCP1 antibody (A03361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (Catalog # A03361) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70 kDa. The expected band size for LCP1 is at 70 kDa.
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