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LCP1 (phospho-Tyr28) rabbit pAb

ORB2304776
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
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Overview

  • Supplier
    Biorbyt
  • Product Name
    LCP1 (phospho-Tyr28) rabbit pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Isotype
    IgG
  • Scientific Description
    LCP1 (phospho-Tyr28) rabbit pAb
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LCP1 using anti-LCP1 antibody (A03361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (Catalog # A03361) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70 kDa. The expected band size for LCP1 is at 70 kDa.
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