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ProductsBack/LCTL Antibody

LCTL Antibody

LS-C754519
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetLCTL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LCTL Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, IF, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID197021
  • Target name
    LCTL
  • Target description
    lactase like
  • Target synonyms
    gammaklotho; KL lactase phlorizin hydrolase; KLG; klotho gamma; klotho/lactase-phlorizin hydrolase-related protein; KLPH; lactase-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LCTL using anti-LCTL antibody (A14882-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCTL antigen affinity purified polyclonal antibody (Catalog # A14882-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCTL at approximately 70 kDa. The expected band size for LCTL is at 65 kDa.
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