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Western blot All lanes: LDAH antibody at 8microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38, 33, 23 kDa Observed band size: 38 kDa
Western blot All lanes: LDAH antibody at 8microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38, 33, 23 kDa Observed band size: 38 kDa
Western blot All lanes: LDAH antibody at 8microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38, 33, 23 kDa Observed band size: 38 kDa

LDAH Antibody

CSB-PA875685LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLDAH
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Overview

  • Supplier
    Cusabio
  • Product Name
    LDAH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID60526
  • Target name
    LDAH
  • Target description
    lipid droplet associated hydrolase
  • Target synonyms
    C2orf43, hLDAH, lipid droplet-associated hydrolase, UPF0554 protein C2orf43, lipid droplet-associated serine hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6V9
  • Protein Name
    Lipid droplet-associated hydrolase
  • Scientific Description
    Serine lipid hydrolase associated with lipid droplets. Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C2orf43/LDAH using anti-C2orf43/LDAH antibody (A13985). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SK-N-SH whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody (Catalog # A13985) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C2orf43/LDAH at approximately 37 kDa. The expected band size for C2orf43/LDAH is at 37 kDa.
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