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Western blot using GeneTex affinity purified anti-LDB1 antibody (GTX23499) shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4oC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
Western blot using GeneTex affinity purified anti-LDB1 antibody (GTX23499) shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4oC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
Western blot using GeneTex affinity purified anti-LDB1 antibody (GTX23499) shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4oC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).

LDB1 antibody

GTX23499
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
DownloadDatasheet
Product group Antibodies
ReactivityHuman
TargetLdb1
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Overview

  • Supplier
    GeneTex
  • Product Name
    LDB1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ELISA: 1:425000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16825
  • Target name
    Ldb1
  • Target description
    LIM domain binding 1
  • Target synonyms
    carboxyl-terminal LIM domain-binding protein 2; CLI; CLIM2; LIM domain-binding factor CLIM2; LIM domain-binding protein 1; NLI; nuclear LIM interactor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP70662
  • Protein Name
    LIM domain-binding protein 1
  • Scientific Description
    The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB 1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203