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LDOC1 Polyclonal Antibody

BS-6543R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Porcine, Rabbit
TargetLDOC1
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Overview

  • Supplier
    Bioss
  • Product Name
    LDOC1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23641
  • Target name
    LDOC1
  • Target description
    LDOC1 regulator of NFKB signaling
  • Target synonyms
    BCUR1, Mar7, Mart7, RTL7, SIRH7, protein LDOC1, Sushi-Ichi retrotransposon homolog 7, breast cancer, up-regulated 1, leucine zipper down-regulated in cancer 1, leucine zipper downregulated in cancer, leucine zipper protein down-regulated in cancer cells, mammalian retrotransposon-derived 7, retrotransposon Gag like 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95751
  • Protein Name
    Protein LDOC1
  • Reactivity
    Canine, Equine, Human, Porcine, Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of LDOC1 using anti-LDOC1 antibody (A09804-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDOC1 antigen affinity purified polyclonal antibody (Catalog # A09804-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDOC1 at approximately 15 kDa. The expected band size for LDOC1 is at 17 kDa.
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