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Lecithin Cholesterol Acyltransferase (LCAT) Polyclonal Antibody

CAU21717
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetLcat
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Overview

  • Supplier
    Biomatik
  • Product Name
    Lecithin Cholesterol Acyltransferase (LCAT) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16816
  • Target name
    Lcat
  • Target description
    lecithin cholesterol acyltransferase
  • Target synonyms
    D8Wsu61e, phosphatidylcholine-sterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase, PAF acetylhydrolase, lecithin-cholesterol acyltransferase Lcat, phospholipid-cholesterol acyltransferase, platelet-activating factor acetylhydrolase
  • Host
    Rabbit
  • Protein IDP16301
  • Protein Name
    Phosphatidylcholine-sterol acyltransferase
  • Scientific Description
    The Lecithin Cholesterol Acyltransferase (LCAT) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LCAT using anti-LCAT antibody (PB9657). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Testis Tissue Lysate at 50ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCAT antigen affinity purified polyclonal antibody (Catalog # PB9657) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCAT at approximately 50 kDa. The expected band size for LCAT is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-LCAT Antibody Picoband(r)PB9657-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetLcat
  • SizePrice