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IHC-P analysis of human prostate tissue using GTX32702 Leptin antibody. Dilution : 1:100
IHC-P analysis of human prostate tissue using GTX32702 Leptin antibody. Dilution : 1:100
IHC-P analysis of human prostate tissue using GTX32702 Leptin antibody. Dilution : 1:100

Leptin antibody

GTX32702
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLEP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Leptin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3952
  • Target name
    LEP
  • Target description
    leptin
  • Target synonyms
    LEPD; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; obese protein; obese, mouse, homolog of; obesity factor; OBS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41159
  • Protein Name
    Leptin
  • Scientific Description
    This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (A00479-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Leptin protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # A00479-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 16KD. The expected band size for Leptin is at 16KD.
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