Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Leptin Polyclonal Antibody
BS-0108R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Mouse, Porcine, Rat
TargetLep
Overview
- SupplierBioss Antibodies
- Product NameLeptin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID16846
- Target nameLep
- Target descriptionleptin
- Target synonymsleptin; ob; obese; obesity factor
- HostRabbit
- IsotypeIgG
- Protein IDP41160
- Protein NameLeptin
- ReactivityBovine, Canine, Equine, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC12352203