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Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Leptin Polyclonal Antibody

BS-0108R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLep
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Leptin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID16846
  • Target name
    Lep
  • Target description
    leptin
  • Target synonyms
    leptin; ob; obese; obesity factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41160
  • Protein Name
    Leptin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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WB analysis of recombinant mouse leptin protein using GTX22095 Leptin antibody. Lane 1 : Reduced recombinant mouse leptin Lane 2 : Protein ladder Lane 3 : Non-reduced recombinant mouse leptin Loading : 0.03 microg Dilution : 1:1000
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Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (A00479-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse small intestine tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # A00479-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 23KD. The expected band size for Leptin is at 18KD.
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