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Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4°C, DAB staining.

Leptin Polyclonal Antibody

BS-0409R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat, Sheep
TargetLEP
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Overview

  • Supplier
    Bioss
  • Product Name
    Leptin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID280836
  • Target name
    LEP
  • Target description
    leptin
  • Target synonyms
    ob, leptin, obese, obesity factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50595
  • Protein Name
    Leptin
  • Reactivity
    Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li F, Zhao S, Guo T, et al. The Nutritional Cytokine Leptin Promotes NSCLC by Activating the PI3K/AKT and MAPK/ERK Pathways in NSCLC Cells in a Paracrine Manner. Biomed Res Int. 2019,2019:2585743. doi: 10.1155/2019/2585743
    Read this paper
  • Chu M, Zhao Y, Feng Y, et al. MicroRNA-126 participates in lipid metabolism in mammary epithelial cells. Mol Cell Endocrinol. 2017,454:77-86. doi: 10.1016/j.mce.2017.05.039
    Read this paper
  • Zhang Y, Liu L, Li C, et al. Correlation analysis between the expressions of leptin and its receptor (ObR) and clinicopathology in endometrial cancer. Cancer Biomark. 2014,14(5):353-9. doi: 10.3233/CBM-140415
    Read this paper

Datasheet

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