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Leukemia Inhibitory Factor (LIF) Polyclonal Antibody

CAU28672
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetLif
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Overview

  • Supplier
    Biomatik
  • Product Name
    Leukemia Inhibitory Factor (LIF) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.44 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16878
  • Target name
    Lif
  • Target description
    leukemia inhibitory factor
  • Target synonyms
    leukemia inhibitory factor, d factor, differentiation-stimulating factor, myeloid leukaemia inhibitory factor
  • Host
    Rabbit
  • Protein IDP09056
  • Protein Name
    Leukemia inhibitory factor
  • Scientific Description
    The Leukemia Inhibitory Factor (LIF) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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WB analysis of various samples using GTX11940 LIF antibody. Lane 1 : rat Intestine tissue lysate at 50ug Lane 2 : rat brain tissue lysate at 50ug Lane 3 : rat spleen tissue lysate at 50ug Lane 4 : rat thymus tissue lysate at 50ug Lane 5 : mouse brain tissue lysate at 50ug Lane 6 : mouse kidney tissue lysate at 50ug Lane 7 : mouse liver tissue lysate at 50ug Dilution : 0.5 microg/mL
Product group Antibodies
References
GeneTex
LIF antibodyGTX11940
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetLif
  • SizePrice
Figure 1. Western blot analysis of LIF using anti-LIF antibody (A01400). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: human Hela cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human Jurkat cell lysates, Lane 8: mouse liver tissue lysates. Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIF antigen affinity purified polyclonal antibody (Catalog # A01400) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIF at approximately 18KD. The expected band size for LIF is at 22KD.
Product group Antibodies
Boster Bio
Anti-LIF Antibody Picoband(r)A01400-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetLif
  • SizePrice