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Western Blot analysis of 3T3 cells using Galectin-1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Galectin-1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Galectin-1 Polyclonal Antibody

LGALS1 Antibody

CSB-PA009814
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetLGALS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LGALS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3956
  • Target name
    LGALS1
  • Target description
    galectin 1
  • Target synonyms
    GAL1, GBP, galectin-1, 14 kDa laminin-binding protein, 14 kDa lectin, HBL, HLBP14, HPL, S-Lac lectin 1, beta-galactoside-binding lectin L-14-I, beta-galactoside-binding protein 14kDa, epididymis secretory sperm binding protein, gal-1, galaptin, lactose-binding lectin 1, lectin, galactoside-binding, soluble, 1, putative MAPK-activating protein PM12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09382
  • Protein Name
    Galectin-1
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (PB9240-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # PB9240-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15 kDa. The expected band size for Galectin 1 is at 15 kDa.
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Lane 1: NIH/3T3 cell lysates probed with Galectin 1 Polyclonal Antibody, Unconjugated (bs-6594R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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