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Western Blot analysis of 3T3 cells using Galectin-8 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Galectin-8 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Galectin-8 Polyclonal Antibody

LGALS8 Antibody

CSB-PA009823
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetLGALS8
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Overview

  • Supplier
    Cusabio
  • Product Name
    LGALS8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3964
  • Target name
    LGALS8
  • Target description
    galectin 8
  • Target synonyms
    Gal-8; galectin-8; galectin-8g; lectin, galactoside-binding, soluble, 8; PCTA1; PCTA-1; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; Po66-CBP; prostate carcinoma tumor antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00214
  • Protein Name
    Galectin-8
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Galectin 8 using anti-Galectin 8 antibody (PB9659). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates Lane 2: rat testicular tissue lysates Lane 3: mouse Neuro-2A whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 8 antigen affinity purified polyclonal antibody (Catalog # PB9659) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 8 at approximately 36KD. The expected band size for Galectin 8 is at 36KD.
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