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Western Blot analysis of A549 cells using LHR Polyclonal Antibody
Western Blot analysis of A549 cells using LHR Polyclonal Antibody
Western Blot analysis of A549 cells using LHR Polyclonal Antibody

LHCGR Antibody

CSB-PA009824
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLHCGR
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Overview

  • Supplier
    Cusabio
  • Product Name
    LHCGR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3973
  • Target name
    LHCGR
  • Target description
    luteinizing hormone/choriogonadotropin receptor
  • Target synonyms
    HHG, LCGR, LGR2, LH/CG-R, LH/CGR, LHR, LHRHR, LSH-R, ULG5, lutropin-choriogonadotropic hormone receptor, hypergonadotropic hypogonadism, lutropin/choriogonadotropin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22888
  • Protein Name
    Lutropin-choriogonadotropic hormone receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of hCG receptor/LHR/LHCGR using anti-hCG receptor/LHR/LHCGR antibody (A01120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hCG receptor/LHR/LHCGR antigen affinity purified polyclonal antibody (Catalog # A01120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hCG receptor/LHR/LHCGR at approximately 78 kDa. The expected band size for hCG receptor/LHR/LHCGR is at 78 kDa.
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