Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LIF antibody (GTX101021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with LIF antibody [N1C3] (GTX101021) diluted at 1:500 and competitor's antibody (sc-1336) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101021/GTX101021_42872_20171006_WB_competitor_watermark_w_23060100_786.webp "Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with LIF antibody [N1C3] (GTX101021) diluted at 1:500 and competitor's antibody (sc-1336) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LIF antibody (GTX101021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LIF antibody
GTX101021
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetLIF
Overview
- SupplierGeneTex
- Product NameLIF antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.07 mg/ml
- ConjugateUnconjugated
- Gene ID3976
- Target nameLIF
- Target descriptionLIF interleukin 6 family cytokine
- Target synonymsCDF, DIA, HILDA, MLPLI, leukemia inhibitory factor, D factor, cholinergic differentiation factor, differentiation inhibitory activity, differentiation-inducing factor, differentiation-stimulating factor, hepatocyte-stimulating factor III, human interleukin in DA cells, melanoma-derived LPL inhibitor
- HostRabbit
- IsotypeIgG
- Protein IDP15018
- Protein NameLeukemia inhibitory factor
- Scientific DescriptionThe protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- García-Gómez P, Golán I, S Dadras M, et al. NOX4 regulates TGFβ-induced proliferation and self-renewal in glioblastoma stem cells. Mol Oncol. 2022,16(9):1891-1912. doi: 10.1002/1878-0261.13200Read this paper
Datasheet
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