Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells. Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed
Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells. Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed
LIF (human) (rec.)
AG-40B-0093
Overview
- SupplierAdipoGen Life Sciences
- Product NameLIF (human) (rec.)
- Delivery Days Customer10
- CertificationResearch Use Only
- Estimated Purity>95%
- Protein IDP15018
- Protein NameLeukemia inhibitory factor
- Scientific DescriptionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF. - Protein. Human LIF (aa 23-202) is fused at the N-terminus to a tag. Source: E. coli. Endotoxin content: <0.05EU/microg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Mouse and human embryonic stem cells. Purity: >95% (SDS-PAGE). LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352202