(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108649/GTX108649_43824_20200117_WB_TPM_watermark_w_23060120_705.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Wild-type (WT) and LIMCH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108649/GTX108649_40002_20181109_WB_KO_watermark_w_23060120_239.webp "Wild-type (WT) and LIMCH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![LIMCH1 antibody [C2C3], C-term detects LIMCH1 protein at stress fiber by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LIMCH1 stained by LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108649/GTX108649_43824_20210702_ICC_IF_w_23060120_679.webp "LIMCH1 antibody [C2C3], C-term detects LIMCH1 protein at stress fiber by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LIMCH1 stained by LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:500.")
(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LIMCH1 antibody [C2C3], C-term
GTX108649
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetLIMCH1
Overview
- SupplierGeneTex
- Product NameLIMCH1 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.53 mg/ml
- ConjugateUnconjugated
- Gene ID22998
- Target nameLIMCH1
- Target descriptionLIM and calponin homology domains 1
- Target synonymsLIMCH1A, LMO7B, LIM and calponin homology domains-containing protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9UPQ0
- Protein NameLIM and calponin homology domains-containing protein 1
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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