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ICC/IF analysis of NTERA-2 cells using GTX83162 Lin28A antibody [6D1F9]. Green : Lin28A Blue: DRAQ5 fluorescent DNA dye Red : Actin filament
ICC/IF analysis of NTERA-2 cells using GTX83162 Lin28A antibody [6D1F9]. Green : Lin28A Blue: DRAQ5 fluorescent DNA dye Red : Actin filament
ICC/IF analysis of NTERA-2 cells using GTX83162 Lin28A antibody [6D1F9]. Green : Lin28A Blue: DRAQ5 fluorescent DNA dye Red : Actin filament

Lin28A antibody [6D1F9]

GTX83162
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetLIN28A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Lin28A antibody [6D1F9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6D1F9
  • Conjugate
    Unconjugated
  • Gene ID79727
  • Target name
    LIN28A
  • Target description
    lin-28 RNA binding posttranscriptional regulator A
  • Target synonyms
    CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A, protein lin-28 homolog A, RNA-binding protein LIN-28, lin-28 homolog A, zinc finger CCHC domain-containing protein 1, zinc finger, CCHC domain containing 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H9Z2
  • Protein Name
    Protein lin-28 homolog A
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIN28A using anti-LIN28A antibody (A01966-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN28A antigen affinity purified polyclonal antibody (Catalog # A01966-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN28A at approximately 28KD. The expected band size for LIN28A is at 28KD.
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