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LIN28A / LIN28 Antibody

LS-C813038
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLIN28A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LIN28A / LIN28 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79727
  • Target name
    LIN28A
  • Target description
    lin-28 homolog A
  • Target synonyms
    CSDD1; LIN28; LIN-28; lin-28A; protein lin-28 homolog A; RNA-binding protein LIN-28; ZCCHC1; zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIN28A using anti-LIN28A antibody (A01966-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN28A antigen affinity purified polyclonal antibody (Catalog # A01966-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN28A at approximately 28KD. The expected band size for LIN28A is at 28KD.
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