Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LINGO1 antibody [C1C3] (GTX118853) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LINGO1 stained by LINGO1 antibody [C1C3] (GTX118853) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX118853/GTX118853_40597_20171115_ICC_IF_R_w_23060519_979.webp "LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LINGO1 stained by LINGO1 antibody [C1C3] (GTX118853) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LINGO1 antibody [C1C3] (GTX118853) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LINGO1 antibody [C1C3]
GTX118853
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetLINGO1
Overview
- SupplierGeneTex
- Product NameLINGO1 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID84894
- Target nameLINGO1
- Target descriptionleucine rich repeat and Ig domain containing 1
- Target synonymsLERN1, LRRN6A, MRT64, UNQ201, leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, leucine rich repeat neuronal 6A, leucine-rich repeat and immunoglobulin domain-containing protein 1, leucine-rich repeat neuronal protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ96FE5
- Protein NameLeucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1
- Scientific DescriptionFunctional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination.
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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