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ProductsBack/Linker For Activation Of T-Cell (LAT) Polyclonal Antibody

Linker For Activation Of T-Cell (LAT) Polyclonal Antibody

CAU28223
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetLat
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Overview

  • Supplier
    Biomatik
  • Product Name
    Linker For Activation Of T-Cell (LAT) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16797
  • Target name
    Lat
  • Target description
    linker for activation of T cells
  • Target synonyms
    36 kDa phospho-tyrosine adapter protein; linker for activation of T-cells family member 1; linker protein; p36-38; pp36
  • Host
    Rabbit
  • Protein IDO54957
  • Protein Name
    Linker for activation of T-cells family member 1
  • Scientific Description
    The Linker For Activation Of T-Cell (LAT) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lat using anti-Lat antibody (A01654-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lat antigen affinity purified polyclonal antibody (Catalog # A01654-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lat at approximately 38 kDa. The expected band size for Lat is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-Lat Antibody Picoband(r)A01654-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetLat
  • SizePrice