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ProductsBack/Lipin 1 antibody [C3], C-term
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE GTX107992 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE GTX107992 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE GTX107992 diluted at 1:1000

Lipin 1 antibody [C3], C-term

GTX107992
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLPIN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Lipin 1 antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23175
  • Target name
    LPIN1
  • Target description
    lipin 1
  • Target synonyms
    PAP1, phosphatidate phosphatase LPIN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14693
  • Protein Name
    Phosphatidate phosphatase LPIN1
  • Scientific Description
    This gene represents a candidate gene for human lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mouse studies suggest that this gene functions during normal adipose tissue development and may also play a role in human triglyceride metabolism. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lipin 1/LPIN1 using anti-Lipin 1/LPIN1 antibody (A02467-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipin 1/LPIN1 antigen affinity purified polyclonal antibody (Catalog # A02467-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipin 1/LPIN1 at approximately 130 kDa. The expected band size for Lipin 1/LPIN1 is at 99 kDa.
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