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Western blot All lanes: LMO1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 50 kDa
Western blot All lanes: LMO1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 50 kDa
Western blot All lanes: LMO1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 50 kDa

LMO1 Antibody

CSB-PA02055A0RB
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetLMO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LMO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4004
  • Target name
    LMO1
  • Target description
    LIM domain only 1
  • Target synonyms
    cysteine-rich protein TTG-1; LIM domain only 1 (rhombotin 1); LIM domain only protein 1; LMO-1; RBTN1; RHOM1; rhombotin-1; T-cell translocation gene 1; T-cell translocation protein 1; TTG1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25800
  • Protein Name
    Rhombotin-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LMO1 using anti-LMO1 antibody (A06093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat brain tissue lysate, lane 2: NIH3T3 whole cell lysates, lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO1 antigen affinity purified polyclonal antibody (Catalog # A06093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO1 at approximately 18KD. The expected band size for LMO1 is at 18KD.
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