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LMO1 Antibody

CAB7561
Assay Genie
TargetLMO1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    LMO1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4004
  • Target name
    LMO1
  • Target description
    LIM domain only 1
  • Target synonyms
    cysteine-rich protein TTG-1; LIM domain only 1 (rhombotin 1); LIM domain only protein 1; LMO-1; RBTN1; RHOM1; rhombotin-1; T-cell translocation gene 1; T-cell translocation protein 1; TTG1
  • Host
    Rabbit
  • Protein IDP25800
  • Protein Name
    Rhombotin-1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LMO1 using anti-LMO1 antibody (A06093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat brain tissue lysate, lane 2: NIH3T3 whole cell lysates, lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO1 antigen affinity purified polyclonal antibody (Catalog # A06093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO1 at approximately 18KD. The expected band size for LMO1 is at 18KD.
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