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WB analysis of Ramos cell lysate using GTX48597 LMO2 antibody.
WB analysis of Ramos cell lysate using GTX48597 LMO2 antibody.
WB analysis of Ramos cell lysate using GTX48597 LMO2 antibody.

LMO2 antibody

GTX48597
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLMO2
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Overview

  • Supplier
    GeneTex
  • Product Name
    LMO2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 microg/ml. IHC-P: 1:250 - 1:500. ChIP assay: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4005
  • Target name
    LMO2
  • Target description
    LIM domain only 2
  • Target synonyms
    cysteine-rich protein TTG-2; LIM domain only protein 2; LMO-2; RBTN2; RBTNL1; RHOM2; rhombotin-2; rhombotin-like 1; T-cell translocation gene 2; T-cell translocation protein 2; TTG2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25791
  • Protein Name
    Rhombotin-2
  • Scientific Description
    LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SMMC-7721 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.
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