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ProductsBack/LOX 1 (OLR1) Rabbit Polyclonal Antibody
Western blot analysis of various lysates using OLR1 Rabbit pAb (TA332415) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Western blot analysis of various lysates using OLR1 Rabbit pAb (TA332415) at 1:500 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 180s.
Western blot analysis of various lysates using OLR1 Rabbit pAb (TA332415) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

LOX 1 (OLR1) Rabbit Polyclonal Antibody

TA332415
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetOLR1
Price on request
100 ul
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More than 130 suppliers
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Overview

  • Supplier
    OriGene
  • Product Name
    LOX 1 (OLR1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4973
  • Target name
    OLR1
  • Target description
    oxidized low density lipoprotein receptor 1
  • Target synonyms
    CLEC8A, LOX1, LOXIN, SCARE1, SLOX1, oxidized low-density lipoprotein receptor 1, C-type lectin domain family 8 member A, hLOX-1, lectin-type oxidized LDL receptor 1, ox LDL receptor 1, oxidized low density lipoprotein (lectin-like) receptor 1, oxidized low-density lipoprotein receptor 1, soluble form, scavenger receptor class E, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78380
  • Protein Name
    Oxidized low-density lipoprotein receptor 1
  • Scientific Description
    Rabbit anti-OLR1 Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
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