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ProductsBack/Anti-LOX 1/Olr1 Antibody

Anti-LOX 1/Olr1 Antibody

ORB623814
Biorbyt
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetOlr1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-LOX 1/Olr1 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    Western blot, 0.25-0.5microg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml, Rat Flow Cytometry (Fixed), 1-3microg/1x106 cells, Rat ELISA, 0.1-0.5microg/ml,. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, FC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID140914
  • Target name
    Olr1
  • Target description
    oxidized low density lipoprotein receptor 1
  • Target synonyms
    LOX-1, Oldlr1, Oldr1, oxidized low-density lipoprotein receptor 1, Lectin-like oxidized low-density lipoprotein receptor-1, lectin-like oxLDL receptor 1, lectin-like oxidized LDL receptor 1, lectin-like oxidized low-density lipoprotein receptor, lectin-type oxidized LDL receptor 1, ox-LDL receptor 1, oxidised low density lipoprotein (lectin-like) receptor 1, oxidized low density lipoprotein (lectin-like) receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO70156
  • Protein Name
    Oxidized low-density lipoprotein receptor 1
  • Scientific Description
    Anti-LOX 1/Olr1 Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Olr1 using anti-Olr1 antibody (A00760-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse smooth muscle tissue lysates, Lane 5: mouse SP2/0 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat smooth muscle tissue lysates, Lane 11: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Olr1 antigen affinity purified polyclonal antibody (Catalog # A00760-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Olr1 at approximately 52 kDa. The expected band size for Olr1 is at 42 kDa.
Product group Antibodies
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Anti-LOX 1/Olr1 Antibody Picoband(r)A00760-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetOlr1
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ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetOlr1
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