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IHC-P analysis of human breast adenocarcinoma tissue using GTX84182 LOX antibody [6B11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human breast adenocarcinoma tissue using GTX84182 LOX antibody [6B11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human breast adenocarcinoma tissue using GTX84182 LOX antibody [6B11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.

LOX antibody [6B11]

GTX84182
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLOX
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Overview

  • Supplier
    GeneTex
  • Product Name
    LOX antibody [6B11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6B11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4015
  • Target name
    LOX
  • Target description
    lysyl oxidase
  • Target synonyms
    AAT10; protein-lysine 6-oxidase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP28300
  • Protein Name
    Protein-lysine 6-oxidase
  • Scientific Description
    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mechanical injury and IL-1beta regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes. Wang C et al., 2020 Aug, Biotechnol Lett
    Read more
  • Hypoxia Inducible Factors Modify Collagen I Fibers in MDA-MB-231 Triple Negative Breast Cancer Xenografts. Goggins E et al., 2018 Feb, Neoplasia
    Read more

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Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
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