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ProductsBack/LOX Rabbit Polyclonal Antibody
Detection of LOX in mouse kidney lysate, ECL detection was 15 sec
Detection of LOX in mouse kidney lysate, ECL detection was 15 sec
Detection of LOX in mouse kidney lysate, ECL detection was 15 sec

LOX Rabbit Polyclonal Antibody

TA301771
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat
TargetLOX
Price on request
50 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    LOX Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4015
  • Target name
    LOX
  • Target description
    lysyl oxidase
  • Target synonyms
    AAT10, protein-lysine 6-oxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28300
  • Protein Name
    Protein-lysine 6-oxidase
  • Scientific Description
    Rabbit Polyclonal Antibody against LOX
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
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