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ProductsBack/LPAR6 / P2RY5 / P2Y5 Antibody (aa85-134, FITC)

LPAR6 / P2RY5 / P2Y5 Antibody (aa85-134, FITC)

LS-C474239
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Mammals, Monkey, Mouse, Primate, Rabbit, Rat
TargetLPAR6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LPAR6 / P2RY5 / P2Y5 Antibody (aa85-134, FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID10161
  • Target name
    LPAR6
  • Target description
    lysophosphatidic acid receptor 6
  • Target synonyms
    ARWH1, HYPT8, LAH3, LPA-6, P2RY5, P2Y5, lysophosphatidic acid receptor 6, G-protein coupled purinergic receptor P2Y5, LPA receptor 6, P2Y purinoceptor 5, RB intron encoded G-protein coupled receptor, oleoyl-L-alpha-lysophosphatidic acid receptor, purinergic receptor 5, purinergic receptor P2Y G protein-coupled protein 5, purinergic receptor P2Y, G-protein coupled, 5
  • Host
    Rabbit
  • Reactivity
    Hamster, Human, Mammals, Monkey, Mouse, Primate, Rabbit, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P2RY5 using anti-P2RY5 antibody (A05725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RY5 antigen affinity purified polyclonal antibody (Catalog # A05725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RY5 at approximately 39 kDa. The expected band size for P2RY5 is at 39 kDa.
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