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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: LPCAT2 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 31 kDa Observed band size: 61 kDa
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: LPCAT2 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 31 kDa Observed band size: 61 kDa
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: LPCAT2 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 31 kDa Observed band size: 61 kDa

LPCAT2 Antibody

CSB-PA013053LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLPCAT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LPCAT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54947
  • Target name
    LPCAT2
  • Target description
    lysophosphatidylcholine acyltransferase 2
  • Target synonyms
    1-acylglycerol-3-phosphate O-acyltransferase 11; 1-acylglycerophosphocholine O-acyltransferase; 1-AGP acyltransferase 11; 1-AGPAT 11; 1-alkenylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acetyl-CoA:lyso-PAF acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 2; acyltransferase-like 1; AGPAT11; AYTL1; LPAAT-alpha; LPC acyltransferase 2; LPCAT-2; lyso-PAF acetyltransferase; LysoPAFAT; lysoPC acyltransferase 2; lysophosphatidic acid acyltransferase alpha; lysophosphatidylcholine acyltransferase 2; lyso-platelet-activating factor (PAF) acetyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L5N7
  • Protein Name
    Lysophosphatidylcholine acyltransferase 2
  • Scientific Description
    Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Also catalyzes the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LPCAT2 using anti-LPCAT2 antibody (A07471-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPCAT2 antigen affinity purified polyclonal antibody (Catalog # A07471-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPCAT2 at approximately 53 kDa. The expected band size for LPCAT2 is at 60 kDa.
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