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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA821685LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA821685LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA821685LA01HU at dilution of 1:100

LPGAT1 Antibody

CSB-PA821685LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLPGAT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LPGAT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9926
  • Target name
    LPGAT1
  • Target description
    lysophosphatidylglycerol acyltransferase 1
  • Target synonyms
    FAM34A, FAM34A1, LPLAT7, NET8, acyl-CoA:lysophosphatidylglycerol acyltransferase 1, 2-acylglycerophosphocholine O-acyltransferase, acyl-CoA:monoacylglycerol acyltransferase LPGAT1, family with sequence similarity 34, member A, lysophospholipid acyltransferase 7, stearoyl-CoA:1-lyso-2-acyl-PE acyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92604
  • Protein Name
    Acyl-CoA:lysophosphatidylglycerol acyltransferase 1
  • Scientific Description
    Lysophosphatidylglycerol (LPG) specific acyltransferase that recognizes various acyl-CoAs and LPGs as substrates but demonstrates a clear preference for long chain saturated fatty acyl-CoAs and oleoyl-CoA as acyl donors. Prefers oleoyl-LPG over palmitoyl-LPG as an acyl receptor and oleoyl-CoA over lauroyl-CoA as an acyl donor.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LPGAT1 using anti-LPGAT1 antibody (A13705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPGAT1 antigen affinity purified polyclonal antibody (Catalog # A13705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPGAT1 at approximately 43 kDa. The expected band size for LPGAT1 is at 43 kDa.
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Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
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