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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA570494(LRIG3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA570494(LRIG3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA570494(LRIG3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

LRIG3 Antibody

CSB-PA570494
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLRIG3
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Overview

  • Supplier
    Cusabio
  • Product Name
    LRIG3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID121227
  • Target name
    LRIG3
  • Target description
    leucine rich repeats and immunoglobulin like domains 3
  • Target synonyms
    LIG3, leucine-rich repeats and immunoglobulin-like domains protein 3, LIG-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UXM1
  • Protein Name
    Leucine-rich repeats and immunoglobulin-like domains protein 3
  • Scientific Description
    Leucine-rich repeats and immunoglobulin-like domains 3 (Lrig3) was identified by microarray analysis among genes that show differential expression during gastrulation in Xenopus laevis. Lrig3 was expressed in the neural plate and neural crest (NC) at neurula stages, and in NC derivatives and other dorsal structures during tailbud stages. A prominent consequence of the morpholino-induced inhibition of Lrig3 expression was impaired NC formation, as revealed by the suppression of marker genes, including Slug, Sox9 and Foxd3.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LRIG3 using anti-LRIG3 antibody (PB10077). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG3 antigen affinity purified polyclonal antibody (Catalog # PB10077) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG3 at approximately 123 kDa. The expected band size for LRIG3 is at 123 kDa.
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