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LRRC47 Antibody (aa533-583)

LS-C762149
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetLRRC47
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LRRC47 Antibody (aa533-583)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:2000 - 1:10000) Immunoprecipitation Range: 2-10 µg/mg lysate
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57470
  • Target name
    LRRC47
  • Target description
    leucine rich repeat containing 47
  • Target synonyms
    leucine-rich repeat-containing protein 47
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC47 using anti-LRRC47 antibody (A14623-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC47 antigen affinity purified polyclonal antibody (Catalog # A14623-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC47 at approximately 68 kDa. The expected band size for LRRC47 is at 63 kDa.
Product group Antibodies
Boster Bio
Anti-LRRC47 Antibody Picoband(r)A14623-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetLRRC47
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Immunohistochemical staining of human cerebral cortex, colon, kidney and testis using Anti-LRRC47 antibody HPA012018 (A) shows similar protein distribution across tissues to independent antibody HPA008512 (B).
Product group Antibodies
Atlas Antibodies
Anti-LRRC47 AntibodyHPA012018
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLRRC47
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