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ProductsBack/LRRC59 Antibody (aa201-250, HRP)

LRRC59 Antibody (aa201-250, HRP)

LS-C452226
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLRRC59
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LRRC59 Antibody (aa201-250, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.65 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID55379
  • Target name
    LRRC59
  • Target description
    leucine rich repeat containing 59
  • Target synonyms
    leucine-rich repeat-containing protein 59; p34; PRO1855; ribosome-binding protein p34
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, colon, liver and lymph node using Anti-LRRC59 antibody HPA030829 (A) shows similar protein distribution across tissues to independent antibody HPA030827 (B).
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Figure 1. Western blot analysis of LRRC59 using anti-LRRC59 antibody (A09874-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (Catalog # A09874-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC59 at approximately 35 kDa. The expected band size for LRRC59 is at 35 kDa.
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Product group Antibodies
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LRRC59 Polyclonal Antibody, Unconjugated (bs-8361R) at 1:200 overnight at 4°C, DAB staining.
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