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ProductsBack/LSM14A antibody [N3C3]

LSM14A antibody [N3C3]

GTX120902
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetLSM14A
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Overview

  • Supplier
    GeneTex
  • Product Name
    LSM14A antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID26065
  • Target name
    LSM14A
  • Target description
    LSM14A mRNA processing body assembly factor
  • Target synonyms
    C19orf13, FAM61A, RAP55, RAP55A, protein LSM14 homolog A, LSM14 homolog A, LSM14A, SCD6 homolog A, RNA-associated protein 55, RNA-associated protein 55A, alphaSNBP, family with sequence similarity 61, member A, hRAP55, hRAP55A, protein SCD6 homolog, putative alpha-synuclein-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8ND56
  • Protein Name
    Protein LSM14 homolog A
  • Scientific Description
    Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Bearss JJ, Padi SK, Singh N, et al. EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics. EMBO Rep. 2021,22(4):e50835. doi: 10.15252/embr.202050835
    Read this paper
  • Di Stefano B, Luo EC, Haggerty C, et al. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. Cell Stem Cell. 2019,25(5):622-638.e13. doi: 10.1016/j.stem.2019.08.018
    Read this paper
  • Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012
    Read this paper

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Figure 1. Western blot analysis of LSM14A using anti-LSM14A antibody (A06546-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM14A antigen affinity purified polyclonal antibody (Catalog # A06546-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.
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