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LSM2 Antibody

CSB-PA013205GA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLSM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LSM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57819
  • Target name
    LSM2
  • Target description
    LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
  • Target synonyms
    C6orf28, G7B, YBL026W, snRNP, U6 snRNA-associated Sm-like protein LSm2, LSM2 U6 small nuclear RNA and mRNA degradation associated, LSM2 homolog, U6 small nuclear RNA associated, protein G7b, small nuclear ribonuclear protein D homolog, snRNP core Sm-like protein Sm-x5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y333
  • Protein Name
    U6 snRNA-associated Sm-like protein LSm2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LSM2 using anti-LSM2 antibody (A07586-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: monkey Cos-7 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM2 antigen affinity purified polyclonal antibody (Catalog # A07586-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM2 at approximately 11KD. The expected band size for LSM2 is at 11KD.
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