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LSM5 Antibody (C-Terminus)

LS-C169823
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLSM5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LSM5 Antibody (C-Terminus)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23658
  • Target name
    LSM5
  • Target description
    LSM5 homolog, U6 small nuclear RNA and mRNA degradation associated
  • Target synonyms
    LSM5 homolog, U6 small nuclear RNA associated; LSM5 U6 small nuclear RNA and mRNA degradation associated; U6 snRNA-associated Sm-like protein LSm5; YER146W
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LSM5 using anti-LSM5 antibody (A10199-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM5 antigen affinity purified polyclonal antibody (Catalog # A10199-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM5 at approximately 12KD. The expected band size for LSM5 is at 12KD.
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