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ProductsBack/LSM5 Antibody (clone AT25F8)

LSM5 Antibody (clone AT25F8)

LS-C755509
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLSM5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LSM5 Antibody (clone AT25F8)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    ELISA, Flo, ICC (1:100), WB (1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT25F8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23658
  • Target name
    LSM5
  • Target description
    LSM5 homolog, U6 small nuclear RNA and mRNA degradation associated
  • Target synonyms
    YER146W, U6 snRNA-associated Sm-like protein LSm5, LSM5 U6 small nuclear RNA and mRNA degradation associated, LSM5 homolog, U6 small nuclear RNA associated
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LSM5 using anti-LSM5 antibody (A10199-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM5 antigen affinity purified polyclonal antibody (Catalog # A10199-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM5 at approximately 12KD. The expected band size for LSM5 is at 12KD.
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