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Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skin tissue, Primary antibody: CSB-PA143897(Ly6a Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skin tissue, Primary antibody: CSB-PA143897(Ly6a Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skin tissue, Primary antibody: CSB-PA143897(Ly6a Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

Ly6a Antibody

CSB-PA143897
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetLy6a
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Overview

  • Supplier
    Cusabio
  • Product Name
    Ly6a Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID110454
  • Target name
    Ly6a
  • Target description
    lymphocyte antigen 6 family member A
  • Target synonyms
    Ly-6A.2, Ly-6A/E, Ly-6E.1, Sca-1, Sca1, TAP, lymphocyte antigen 6A-2/6E-1, T-cell-activating protein, lymphocyte antigen 6 complex, locus A, stem cell antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05533
  • Protein Name
    Lymphocyte antigen 6A-2/6E-1
  • Scientific Description
    Sca-1 is an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family. Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSCs in mice of both Ly-6 haplotypes. Sca-1-positive HSCs can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Sca1/Ly6A/E using anti-Sca1/Ly6A/E antibody (A30403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sca1/Ly6A/E antigen affinity purified polyclonal antibody (Catalog # A30403) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sca1/Ly6A/E at approximately 14KD. The expected band size for Sca1/Ly6A/E is at 14KD.
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