Non-transfected (–) and transfected (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637770/GTX637770_44907_20230929_IHC-P_23091901_783.webp "LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![LY6E antibody [HL1933] detects LY6E protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637770/GTX637770_44907_20231110_ICC_IF_23121122_564.webp "LY6E antibody [HL1933] detects LY6E protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637770/GTX637770_T-44844_20241220_WB_TPM_watermark_24122400_230.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Non-transfected (–) and transfected (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LY6E antibody [HL1933]
GTX637770
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLY6E
Overview
- SupplierGeneTex
- Product NameLY6E antibody [HL1933]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1933
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4061
- Target nameLY6E
- Target descriptionlymphocyte antigen 6 family member E
- Target synonymsRIG-E, RIGE, SCA-2, SCA2, TSA-1, lymphocyte antigen 6E, ly-6E, lymphocyte antigen 6 complex, locus E, retinoic acid induced gene E, retinoic acid-induced gene E protein, stem cell antigen 2, thymic shared antigen 1
- HostRabbit
- IsotypeIgG
- Protein IDQ16553
- Protein NameLymphocyte antigen 6E
- Scientific DescriptionThis gene belongs to the human Ly6 gene family and encodes a glycosylphosphatidyl-inositol (GPI)-anchored cell surface protein. The protein plays an important role in T cell physiology, oncogenesis and immunological regulation. The protein is also involved in modulation of viral infection by coronaviruses, SARS-CoV, MERS-CoV and SARS-CoV-2. [provided by RefSeq, Aug 2021]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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