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WB analysis of Jurkat, HepG2, and LOVO cell lysates using GTX87852 LYAR antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat, HepG2, and LOVO cell lysates using GTX87852 LYAR antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat, HepG2, and LOVO cell lysates using GTX87852 LYAR antibody. The lane on the right is blocked with the synthesized peptide.

LYAR antibody

GTX87852
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetLYAR
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Overview

  • Supplier
    GeneTex
  • Product Name
    LYAR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55646
  • Target name
    LYAR
  • Target description
    Ly1 antibody reactive
  • Target synonyms
    ZC2HC2, ZLYAR, cell growth-regulating nucleolar protein, Ly1 antibody reactive homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NX58
  • Protein Name
    Cell growth-regulating nucleolar protein
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LYAR using anti-LYAR antibody (A09986-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYAR antigen affinity purified polyclonal antibody (Catalog # A09986-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYAR at approximately 44 kDa. The expected band size for LYAR is at 44 kDa.
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