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LYPD3 Recombinant Antibody

BSM-60286R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLYPD3
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Overview

  • Supplier
    Bioss
  • Product Name
    LYPD3 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-1000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:50-100), IF(IF=1:20-100), ICC/IF(ICC/IF=1:20-50)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27076
  • Target name
    LYPD3
  • Target description
    LY6/PLAUR domain containing 3
  • Target synonyms
    C4.4A, ly6/PLAUR domain-containing protein 3, 2310061G07Rik, GPI-anchored metastasis-associated protein C4.4A homolog, GPI-anchored metastasis-associated protein homolog, MIG-C4, matrigel-induced gene C4 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95274
  • Protein Name
    Ly6/PLAUR domain-containing protein 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LYPD3 using anti-LYPD3 antibody (A08396). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPD3 antigen affinity purified polyclonal antibody (Catalog # A08396) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPD3 at approximately 75 kDa. The expected band size for LYPD3 is at 36 kDa.
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