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LZIC antibody detects LZIC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LZIC protein stained by LZIC antibody (GTX120288) diluted at 1:500. Blue: Hoechst 33342 staining.
LZIC antibody detects LZIC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LZIC protein stained by LZIC antibody (GTX120288) diluted at 1:500. Blue: Hoechst 33342 staining.
LZIC antibody detects LZIC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LZIC protein stained by LZIC antibody (GTX120288) diluted at 1:500. Blue: Hoechst 33342 staining.

LZIC antibody

GTX120288
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLZIC
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Overview

  • Supplier
    GeneTex
  • Product Name
    LZIC antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84328
  • Target name
    LZIC
  • Target description
    leucine zipper and CTNNBIP1 domain containing
  • Target synonyms
    protein LZIC, leucine zipper and CTNNBIP1 domain-containing protein, leucine zipper and ICAT homologous domain-containing protein, leucine zipper domain and ICAT homologous domain containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WZA0
  • Protein Name
    Protein LZIC
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LZIC using anti-LZIC antibody (A15087). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat thymus tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse thymus tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LZIC antigen affinity purified polyclonal antibody (Catalog # A15087) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LZIC at approximately 21 kDa. The expected band size for LZIC is at 21 kDa.
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