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ProductsBack/mAChR M2 (M2-2-B3) Alexa Fluor(r) 680

mAChR M2 (M2-2-B3) Alexa Fluor(r) 680

SC-33712AF680
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHRM2
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    mAChR M2 (M2-2-B3) Alexa Fluor(r) 680
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 680
  • Gene ID1129
  • Target name
    CHRM2
  • Target description
    cholinergic receptor muscarinic 2
  • Target synonyms
    HM2, muscarinic acetylcholine receptor M2, 7TM receptor, acetylcholine receptor, muscarinic 2, muscarinic M2 receptor
  • Host
    Rat
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHRM2 using anti-CHRM2 antibody (A02733). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat brain tissue lysates, lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM2 antigen affinity purified polyclonal antibody (Catalog # A02733) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRM2 at approximately 52KD. The expected band size for CHRM2 is at 52KD.
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